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. 2022 May 30;13:912193. doi: 10.3389/fimmu.2022.912193

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Copyright © 2022 Jerome, Atkinson, McVey Moffatt, Sepeda, Segal and Sas

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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scRNA seq characterization of neutrophils. (A) scRNA seq of purified blood, 4 hour, and 3 day neutrophils unbiased cluster formation based on differentially expressed genes in each cluster. (B) Heat map of the top 5 cluster defining genes for each cluster. (C) Overlay of neutrophil type on the defined UMAP clusters from (A) showing the location of blood neutrophils, 4 hour, and 3 day i.p zymosan stimulated neutrophils. (D) Pie charts illustrating the percentage of total neutrophils in each cluster for blood, 4 hour, and 3 day neutrophils. (E) UMAP clustering analysis demonstrating the cluster distribution of all cells from each neutrophil group (blood, 4 hour, and 3 day) within the total cellular analysis of all cell groups combined.