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. Author manuscript; available in PMC: 2022 Jun 14.
Published in final edited form as: Sci Transl Med. 2020 Nov 25;12(571):eabc6659. doi: 10.1126/scitranslmed.abc6659

Fig. 3. Ad5-3M virus is resistant to natural IgM- and complement-mediated opsonization and inactivation in mouse and human sera.

Fig. 3.

(A) ELISA measurement of natural IgM binding to the indicated viruses in mouse plasma. Detection of deposition of complement components C4 (B) and C3 (C) on the surface of the virions for indicated viruses in mouse serum using ELISA (n = 8-12). (D) Detection of deposition of human complement C3 on the surface of the virions for indicated viruses in human serum using ELISA (n = 4). (E and F) Virus inactivation in raw serum. Indicated viruses were incubated with 90% raw mouse serum (E) (n = 8), and human serum (F) containing low amounts of neutralizing antibodies (n = 8). Virus infectivity after incubation with serum was normalized to virus infectivity with no serum treatment. The p-values of one-way ANOVA with multiple comparison adjustments are shown above the bars. Bars show mean ± SD. The color of the p-value number indicates the partner for multiple comparison tests. N. S. – not significant; p > 0.05. The methodology and statistical details are as described in Table S1 and Methods.