TABLE 2.

Information relevant to FISH oligonucleotides used in this study

Probe	Sequence (5′–3′)	rRNA target sitea	Specificity	% Formamide	Reference
EUB338	GCTGCCTCCCGTAGGAGT	16S, 338–355	Bacteria	20	3
ALF1b	CGTTCG(C/T)TCTGAGCCAG	16S, 19–35	α Proteobacteria	20	29
BET42a	GCCTTCCCACTTCGTTT	23S, 1027–1043	β Proteobacteria	35	29
BONE23a	GAATTCCATCCCCCTCT	16S, 663–679	β-1 Proteobacteria	35	2
BTWO23a	GAATTCCACCCCCCTCT	16S, 663–679	Competitor for BONE23a	35	2
GAM42a	GCCTTCCCACATCGTTT	23S, 1027–1043	γ Proteobacteria	35	29
HGC69a	TATAGTTACCACCGCCGT	23S, 1901–1918	Actinobacteria	25	34
CF319	TGGTCCGTGTCTCAGTAC	16S, 319–336	Cytophaga-Flavobacterium	35	29
PAO462	CCGTCATCTACWCAGGGTATTAAC	16S, 462–485	PAO clusterb	35	This study
PAO651	CCCTCTGCCAAACTCCAG	16S, 651–668	PAO clusterb	35	This study
PAO846	GTTAGCTACGGCACTAAAAGG	16S, 846–866	PAO clusterb	35	This study
Rc988	AGGATTCCTGACATGTCAAGGG	16S, 988–1009	“Rhodocyclus group”b	NDc	This study

^arRNA, E. coli numbering (12). 

^bSee Fig. 3. 

^cND, not determined.