FIG 1.

Heat map of Listeria MICs to BC. Each row is specific to an individual parent strain of Listeria. For example, row 1 of the heat map corresponds to strain FSL S11-0076, which is a strain of L. seeligeri. “Parent MIC” indicates the initial MIC obtained for a given Listeria strain; “BC passaged MIC” indicates the MIC obtained through serial passaging of a strain in increasing concentrations of BC; “MIC of adapted isolate A” and “MIC of adapted isolate B” indicate the MIC obtained from two isolates obtained from serial passage experiments that were substreaked seven times onto BHI agar. Blue filled circles represent the presence of a BC resistance gene (bcrABC, qacH) in the Listeria parent strain. FSL S11-0256 and FSL S11-0060 showed loss of bcrABC in their corresponding adapted isolate A, and FSL S11-0256 and FSL R9-9884 showed loss of bcrABC in their corresponding adapted isolate B (an asterisk is used to identify these parent strains that showed loss of bcrABC in one of their respective adapted isolates). Blue filled triangles represent the presence of a nonsynonymous mutation in fepR in adapted isolate A.