TABLE 4.

Mutations detected in fepR for adapted isolates of L. monocytogenes or Listeria spp.^a

Adapted isolate ID	Species	Total no. of SNPs in adapted isolate	Mutation detected in fepRb	Mutation typec in fepR	BioProject accession no. or SRA ID of parent straind
FSL H9-0080	L. monocytogenes	0	Deletion	Frameshift	PRJNA761983
FSL H9-0082	L. monocytogenes	1	SNP	Missense	SRR9019233
FSL H9-0095	L. monocytogenes	2	SNP	Nonsense	SRR1027706
FSL H9-0098	L. monocytogenes	2	SNP	Missense	PRJNA761983
FSL H9-0100	L. monocytogenes	4	SNP	Nonsense	SRR12696480
FSL H9-0097	L. monocytogenes	1	SNP	Nonsense	SRR9019214
FSL H9-0105	L. monocytogenes	1	Deletion	Frameshift	SRR9019309
FSL H9-0111	L. innocua	2	SNP	Nonsense	PRJNA761983
FSL H9-0112	L. innocua	4	SNP	Nonsense	PRJNA761983
FSL H9-0122	L. seeligeri	1	SNP	Missense	PRJNA761983
FSL H9-0131	L. seeligeri	2	SNP	Nonsense	PRJNA761983
FSL H9-0132	L. marthii	2	SNP	Missense	PRJNA761983
FSL H9-0134	L. marthii	1	SNP	Missense	PRJNA761983
FSL H9-0137	L. welshimeri	0	Deletion	Frameshift	PRJNA761983
FSL H9-0144	L. ivanovii	1	SNP	Nonsense	PRJNA761983
FSL H9-0145	L. ivanovii	2	SNP	Nonsense	PRJNA761983

^aMutations were identified by comparison of the whole-genome sequences of parent strains and adapted isolates.

^bfepR encodes a transcriptional regulator in the TetR family of transcriptional regulators (GenBank accession no. WP_010991061.1). SNP, single nucleotide polymorphism detected in fepR; deletion, deletion of a single nucleotide in fepR.

^cEither missense (SNP confers amino acid shift in FepR protein), nonsense (either SNP confers a premature stop codon in FepR), or deletion (confers a frameshift in FepR).

^dWhole-genome sequences of all adapted isolates can be found under BioProject accession number PRJNA761983. The parent strain ID that corresponds to each adapted isolate ID is shown in Table 1.