TABLE 2.
Primers used in this study
| Primer name | Sequence (5′–3′)a | Purpose |
|---|---|---|
| P1-F | TAGTCCTGCACCCACTTCAGGGCCG | ΔmsrA construct |
| P1-R | TCGGTCTCCATGCGAGCTCGAATTCGATAGGGACCGGAAAGCGGATTTAA | |
| P2-F | ATCCGCTTTCCGGTCCCTATCGAATTCGAGCTCGCATGGAGACCGA | |
| P2-R | TCGGTCTGCCACAGCACCCGCATTATTTGCCGACTACCTTGGTGAT | |
| P3-F | ACCAAGGTAGTCGGCAAATAATGCGGGTGCTGTGGCAGACCGAGGC | |
| P3-R | GCTCGCACAGCGTGGTGTATCTGGC | |
| P4-F | TCCAGCACACTGGCGGCCGTTACTAGTTCAGGAACCAGCCGTCGTCA | msrA_com construct |
| P4-R | GCATGCCTGCAGGTCGAATCGGATCCGCTCGGTGAAGACCCGGGGG | |
| P5-F | CACCGCTTAACATCACCCCT | ΔDsrO construct |
| P5-R | TGCTCGATGAGTTTTTCTAAGGATAACTTTGTTTTACGAGCGTT | |
| P6-F | CGTAAAACAAAGTTATCCTTAGAAAAACTCATCGAGCATCAAATG | |
| P6-R | CCTGGTTCAAACAACGAAAACATGGAGACCGAGGGCCCTTGACA | |
| P7-F | AAGGGCCCTCGGTCTCCATGTTTTCGTTGTTTGAACCAGGTGCA | |
| P7-R | GGGGATGAACGGCATTCTCT | |
| P8-F | TCCAGCACACTGGCGGCCGTTACTAGTCCACCCTCCCCGACGCCCTGCACG | DsrO_com construct |
| P8-R | GCATGCCTGCAGGTCGAATCGGATCCCGCCGTCCAGCAAACAAAAAACG | |
| msrA RT-F | CGCCGCACAATAGCAGCATG | qRT-PCR for msrA expression pattern |
| msrA RT-R | AGCGGTAATCGGGATTGGGCA | |
| DsrO RT-F | ACAGGTCTACGTTTCACTGTGGATA | qRT-PCR for msrA expression pattern |
| DsrO RT-R | CGCCGTCCAGCAAACAAAAAACGCT | |
| 16s RT-F | ATTCCTGGTGTAGCGGTG | qRT-PCR for 16s rRNA |
| 16s RT-R | ATTCCTGGTGTAGCGGTG | |
| msrA SP1 | TCAGCGGAGTTTGTCGCCGTAGTACTGGCGCAGCT | 5′ RACE for msrA |
| msrA SP2 | CCGAGCTGCTCGATCATTTCGCGGGCCGTCTGTTC | |
| msrA SP3 | AGCGGTAATCGGGATTGGGCACCGTACCGCCGATG | |
| DsrO SP1 | CGCCGTCCAGCAAACAAAAAACGCTGAACAGTCAT | 5′ RACE for DsrO |
| DsrO SP2 | CAGCAAACAAAAAACGCTGAACAGTCATGCTTGAAAATCCGC | |
| DsrO SP3 | AAAAACGCTGAACAGTCATGCTTGAAAATCCGCTTCAAAAGTT |
a
The underlining indicates the restriction enzyme cutting site of Spe1(P4/P8-F) and BamHI(P4/P8-R).