FIGURE 3.

Multiple sequence alignment (MSA) of human receptor binding domains (RBDs) from COVID-19 and N-terminal domain (NTD) from MHV-1 are shown in (B), which confirms that both these domains share less than 12% sequence similarity, which suggests that both these structures are dissimilar. Moreover, structural alignment of the RBDs and NTDs of viral spike proteins showed a root mean square deviation (RMSD) of 17.4 Å (A), which also strongly supports the structural dissimilarity, but they hold the β-sheets in their core structure. Interesting to note, the investigation of both human receptor binding sites by the creation of an electrostatic surface diagram revealed that the structures are dissimilar, but receptor binding sites of both the structures have almost similar, highly hydrophobic binding environments (C,D). Hence the ability of both these domains to strongly bind with SPIKENET.