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. Author manuscript; available in PMC: 2022 Jun 14.
Published in final edited form as: Arthritis Rheumatol. 2021 Apr 20;73(6):943–954. doi: 10.1002/art.41611

Figure 1:

Figure 1:

Expression of GBP-5 is significantly higher in RA and IFN-γ preferentially induces a robust GBP-5 expression to suppress IL-1β-induced chemokine production in RASFs. A, qRT-PCR analysis of human RASTs and NLSTs showed a significant increase in GBP5 expression in RASTs compared to NLSTs. B, Expression of GBP-5 protein is observed in RASTs when compared to NLST levels. The expression of GBP-5 at the protein level is upregulated in the joint homogenates of AIA rats compared to naïve rats. C, IL-1β induces time-dependent expression of GBP-5 protein that peaks around 24 h. D, IL-1β (10 ng/ml) or TNF-α (20 ng/ml) induce GBP-5 protein and mRNA expression which is further amplified by IFN-γ. E, IFN-γ significantly downregulates IL-1β-induced IL-6, IL-8, MMP1, and ENA-78/CXCL5 production in RASFs. The results are presented as mean ±SEM. #p<0.05 or ##p<0.01 vs NS; *p<0.05 or **p<0.01 vs IL-1β.