Figure 2:

Loss of GBP-5 exacerbates IL-1β-induced inflammatory and tissue destructive genes in human RAST. A, A heat map of 3315 differentially modulated genes is presented based on t-test qualification. Red represents lower expression; green represents higher expression. B, Network of enriched GO terms for upregulated and downregulated genes where the node sizes represent the proportion of the number of the gene into a specific term. Different colors were selected for cluster ID. C, IPA deciphers the role of GBP-5 in IL-6 signaling pathway. D, Co-stimulation of human RASFs with IL-6 (10 or 100 ng/ml) and IL-6R (100 ng/ml) upregulates the expression of GBP-5 and p-STAT3 (Tyr⁷⁰⁵). E, mgp130 is upregulated by IL-1β stimulation for 24 h and further augmented upon GBP-5 knockdown in both constitutive and IL-β-treated RASFs. F, Overexpression of GBP-5 was achieved by lentiviral delivery method followed by IL-1β stimulation for 24 h. Exogenous GBP-5 reduced the expression of mgp130 in human RASFs. The values presented in the graphs are mean ±SEM of three independent experiments. *p<0.05 NC versus siRNA treatment.