FIG. 2.

Ethidium bromide-stained agarose gel of PCR amplification products obtained with the group I primers. (A) Amplification of chitinase genes from microbial DNA collected from the coastal Pacific Ocean. Lanes: 1, molecular size markers; 2, PCR with bacterial community DNA; 3, PCR with bacterial community DNA concentrated by ultrafiltration; 4, PCR with Alteromonas sp. strain 0-7 DNA; 5, no-template control. (B) Amplification of chitinase genes from microbial DNA collected from the Delaware Bay estuary. Lanes: 1, molecular size markers; 2, PCR with bacterial community DNA; 3, PCR with Alteromonas sp. strain 0-7 DNA; 5, no-template control.