Figure 5.

Knockdown of GIPR in the ACC blocked the analgesic and anxiolytic effects of D-Ala²-GIP. (A) The schedule of the experimental design. (B) The level of GIPR was knocked down in the ACC. GIPR shRNA transfection was shown by green fluorescent protein in coronal section. Scale bar: 100 μm. (C, D) GIPR shRNA transfection abolished the analgesic effect of D-Ala²-GIP. (E–J) GIPR knockdown abolished the effect of D-Ala²-GIP in OF test (E–G) and in EPM test (H–J). n = 12 in each group except for the groups of (B) (n = 6). Totally, 48 mice were used in this section. NC means Negative Control; GIPR-KD means GIPR knockdown; D-Ala² means D-Ala²-GIP; Pro³ means Pro³-GIP. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, ^**** p < 0.0001.