Figure 8.

Effects of GIPR activation on the levels of synaptic plasticity-related proteins in the ACC. (A) Representative Western blot analysis of GluN2B, p-GluN2B-T1472 and p-GluN2B-S1303. (B–D) Systemic administration of D-Ala²-GIP reversed the up-regulation of above proteins in the ACC of model mice, and Pro³-GIP inhibited the effects of D-Ala²-GIP. (E) Representative Western blot analysis of p-CREB and CREB. (F) D-Ala²-GIP inhibited the increase of p-CREB/CREB ratio, which was abolished by Pro³-GIP. (G) Representative Western blot analysis of GluA1, p-GluA1-S831 and p-GluA1-S845. (H–J) D-Ala²-GIP treatment reversed the up-regulations of GluA1, p-GluA1-S831 and p-GluA1-S845 in the ACC of model mice, and Pro³-GIP blocked the effects of D-Ala²-GIP. n = 6 in each group. Totally, 24 mice were used in this section. D-Ala² means D-Ala²-GIP; Pro³ means Pro³-GIP. ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, ^**** p < 0.0001.