Figure 5.

Effects of PINE on LPS-induced iNOS and COX-2 protein levels in LPS-stimulated RAW264.7 macrophages. Western blotting analysis for iNOS, COX-2 and β-actin levels. Cells were pre-treated with PINE (25, 50 or 100 μg/mL) for 1 h and then treated with LPS (1 μg/mL) for 12 h. Dexamethasone (Dexa; 10 μg/mL) was used as the reference compound. The results were analysed using Chemidoc XRS and Quantity One. Experiments were repeated four times. Significantly different from the control group (^##p < 0.01 and ^###p < 0.001); significantly different from the stimulation group (*p < 0.05 and **p < 0.01).