Figure 6.

Effects of PINE on arachidonic acid-induced ear oedema in mice. (A) PINE (0.1, 0.3 or 1 mg/ear in ethanol) was applied to the inside of the left ears of ICR mice and given 10 min to absorb. Arachidonic acid (AA; 2 mg/ear dissolved in acetone) was applied for 30 min to the left ears. Dexamethasone (Dexa; 0.1 mg/ear) was applied as the reference compound to the left ear in the Dexa group. Ear thickness was measured using the micro-engineer metre. (B) Representative images of mouse ears and (C) H&E staining for each experimental group (magnification, ×10; scale bar, 100 μm). (D) MPO activity was measured in the ear tissues via colorimetry based on the absorbance at 620 nm. (E) The MDA level was quantified in the ear tissues via the TBARS method. Each value represents the mean ± SD of three separate experiments. Significantly different from the AA treatment group (*p < 0.05, **p < 0.01 and ***p < 0.001). Significantly different from the control (^##p < 0.01 and ^###p < 0.001).