FIGURE 2.

ADF improved endothelium-dependent vasorelaxation without affecting endothelium-independent vasorelaxation or phenylephrine-induced vasoconstriction of small mesenteric arteries (SMA) in type 2 diabetic mice. (A) Acetylcholine (ACh)-induced endothelium-dependent vasorelaxation of SMA was impaired in Lepr^db mice. Twelve weeks of ADF in Lepr^db (Lepr^db + ADF) restored endothelium-dependent vasorelaxation back to the level of m Lepr^db control mice. (B) Incubation with the nitric oxide synthase inhibitor (L-NAME) largely attenuated ADF-induced improvement of endothelial function in diabetic mice. (C) Sodium Nitroprusside (SNP)-induced endothelium-independent vasorelaxation of SMA was comparable among groups. (D) Phenylephrine (PE)-induced vasoconstriction of SMA was comparable among groups. Data represent mean ± SEM. n = 5 mice per group with 1–2 rings per mouse. *P < 0.05 vs. m Lepr^db control mice, # p < 0.05 vs. Lepr^db diabetic mice.