Table 1.
Primers used in this study
| qPCR Primers | ||
|---|---|---|
| Target Name | Forward Sequence | Reverse Sequence |
| GAPDH | GGAGCGAGATCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG |
| ATG12 | TAGAGCGAACACGAACCA | CACTGCCAAAAACACTCAT |
| VAPA | GAGATGCGTATTTGAAATGCCC | TCTCTCAGGTGCCGATTTTCT |
| VAPB | AGATGGACTGCGGATGAGGAA | CAGTTGGGGCTAATGCTGAAA |
| OSBP1 | CTACCAGCGGCGATGGTTC | ACCACGGCAGGTATGTCTCAT |
| OSBPL4 | TCGTCGCTGTTCACGGTTG | CGGACACAGGTTCCGATCTTG |
| OSBPL1A | TCCGAAGAAAAAGACTGTGGTG | CAGTTAGGCGCTGTAGGAAGC |
| OSBPL2 | CAGAGGCAAATCAGAAAGTCACG | TCCCCAGTTTTCCCAATCCTA |
| OSBPL3 | TTGGTGTGTCCCAAAAATTGGT | TCCTGGGTGTAATTCATCTCCC |
| OSBPL5 | CTCCGAGTCAGATGGTCGC | TGCCCAGTCTCAGTAGGCT |
| OSBPL6 | AAATAGGCCAAACCAATGTCCA | TCTGAATATCGAGTGGTGCCTT |
| OSBPL7 | CAGAGGGTGTCATGCCTGAG | CGGGTTGTTGCATAATGAAGGA |
| OSBPL8 | TCCTCATAGCCAGGGTTTTGA | AGGATCTGTGATTGTACTGAGCA |
| OSBPL9 | CCAGAACCTGTTCAGTTGTGT | ATGATGTCCCAAGGTAGCCAA |
| OSBPL10 | GGGCGTGCTCAGCAAATAC | ATGCCAGCCTCGAAATCCAG |
| OSBPL11 | CTTGCAGGAGCTGTAATATCACC | CTGTCGCTCTTTTGCATCTGTA |
| Cloning Primers | ||
| Construct | Forward Sequence | Reverse Sequence |
| GFP-OSBPL7 | TATAAAGCTTACATGGACTTCCAAGAG | TTAAGTCGACCTACCAGAGCACGGCCCCATCCAT |
| GFP-OSBPL11 | TATAAAGCTTACATGCAGGGGGGTGAACCAGTG | TTAAGTCGACTCACTCTGCTGGTTGTGTTGT |
| GFP-OSBPL9 (anneals vector) | CTGAAACGTCTTGGTGCTGCCAAGCATTAGTAGATAACTGATCATAATCAGCCATACCACATTTGT | GCTCAGCGGCCCTTCCATGATGGACGCCATGCTTGAGCTCGAGATCTGAGTCCGGCCGGA |
| GFP-OSBPL9 (anneals insert) |
TCCGGCCGGACTCAGATCTCGAGCTCAAGCATGGCGTCCATCATGGAAGGGCCGCTGAGC | GTGGTATGGCTGATTATGATCAGTTATCTACTAATGCTTGGCAGCACCAAGACGTTTCAG |
| GFP-OSBPL10 (anneals vector) |
TACTTCAATCCCCTCTGGAAAGCACACTGATAGATAACTGATCATAATCAGCCATACCACATTTGT | GCCGTCTGTGCCCTGGACTGCCCTCTCCATGCTTGAGCTCGAGATCTGAGTCCGGCCGGA |
| GFP-OSBPL10 (anneals insert) |
TCCGGCCGGACTCAGATCTCGAGCTCAAGCATGGAGAGGGCAGTCCAGGGCACAGACGGC | GTGGTATGGCTGATTATGATCAGTTATCTATCAGTGTGCTTTCCAGAGGGGATTGAAGTA |
All DNA sequences are written 5’ to 3’.