Figure 3.

Targeting of L.m.-containing phagosomes by PINCA enhances their fusion with lysosomes. BMDM or PM were infected with L.m. for the indicated periods of time. (A and B) Colocalization of L.m. with LC3, lysosomal fluid phase markers and ACTB was analyzed by immunofluorescence microscopy in PM (A) or BMDM (B) from GFP-LC3 transgenic WT or cybb^−/- mice. Where indicated, ACTB⁺ L.m. were excluded from analysis to enable precise comparison of the acquisition of lysosomal fluid phase markers by LC3⁺ vs. LC3⁻ L.m.-containing phagosomes. (C) ACTB⁺ L.m. in WT and atg7^−/- BMDM at 5 h after infection were quantified by immunofluorescence microscopy. (D) Bacterial burden of WT and atg7^−/- BMDM was determined by plating on blood agar plates. Data are shown as mean ± SEM of three (A-C) or six (D) independent experiments. Representative micrographs from 5 h after infection are shown. Scale bar: 4 µm. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.