Figure 6.

Enhancing ROS production through CYBB by priming with pro-inflammatory cytokines endows BMDM with the ability to target L.m. by LAP. WT and cybb^−/- BMDM were either left untreated or stimulated for 48 h with TNF or for 24 h with IFNG and then infected with wt (A) or Δhly (B) L.m. for the indicated periods of time. LC3⁺ L.m. were quantified by immunofluorescence microscopy. Data are shown as mean ± SEM of at least three independent experiments. Representative micrographs from 1 h after infection are shown. Scale bar: 4 µm. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.