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. 2021 Sep 14;18(5):1049–1061. doi: 10.1080/15548627.2021.1968228

Figure 2.

Figure 2.

Identification of transcription factors contributing to AAD-induced FAM134B2. (A) Transcription factors (TF) induced in HeLa cells treated with HisOH. HeLa cells (N = 3) were treated with DMEM containing 2 mM HisOH or without HisOH (Ctrl) for 6 h. Total RNA was subjected to RNA-seq (Table S2). (B) Effect of ISR inhibition on induced TF mRNA expression by HisOH. HeLa cells (N = 3) were treated with 200 nM ISRIB for 2 h and then treated with 2 mM HisOH for 6 h. (C) HisOH-induced TF mRNA expression in HeLa cells treated with EBSS, Leu-deficient DMEM or complete DMEM (CM). (D) TF mRNA expression in HeLa cells treated with RNAi. (E) FAM134B2 mRNA expression in HeLa cells treated with siRNAs. HeLa cells were treated with 10 nM RNAi in the presence of RNAi Max for 16 h and then treated with 2 mM HisOH for 6 h. *P < 0.05 vs. Scramble siRNA (Scr).