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. 2021 Sep 14;18(5):1049–1061. doi: 10.1080/15548627.2021.1968228

Figure 6.

Figure 6.

NR4A1 activates FAM134B2-mediated reticulophagy. (A) Immunofluorescence of FAM134B2 (green), LAMP1 (magenta) and FLAG (blue) in mCherry-APOC3 (red) with control plasmid or FLAG-NR4A1 (F-NR4A1) overexpressed HeLa FAM134B2 KO cells treated with 200 nM bafilomycin A1 for 6 h. Arrow indicates mCherry-APOC3 and FAM134B2+RETREG1-positive LAMP1. (B) Immunofluorescence of FAM134B2+RETREG1 (green), LAMP1 (magenta) and mTagBFP2-NR4A1 (blue) in mScarlet-RTN2 (red) with control plasmid or mTagBFP2-NR4A1 overexpressed HeLa FAM134B2-RETREG1 dKO cells treated with 200 nM bafilomycin A1 for 6 h. Arrow indicates mScarlet-RTN2 and FAM134B2+RETREG1-positive LAMP1. Scale: 10 μm, and 2 μm in inset images. Percentage of (C) mCherry-APOC3 and Lysosome double positive, (D) FAM134B2+RETREG1 and lysosome double positive, and (E) mCherry-APOC3-FAM134B2-lysosome triple positive in control vector or FLAG-NR4A1 expressed HeLa WT or FAM134B2 KO (B2 KO) cells treated with bafilomycin A1 for 6 h (n = 12). Quantifications of the RTN2 experiment are shown in Figure S3A–C. *P < 0.05.