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. 2000 Mar;66(3):1223–1227. doi: 10.1128/aem.66.3.1223-1227.2000

FIG. 1.

FIG. 1

Alignment of amino acid sequences of reported pyruvate carboxylases and products of prokaryote gene fragments with the same functional domains. (A) Biotin carboxylase domain; (B) biotin binding domain. Pycl.Sc, S. cerevisiae Pyc1 (33); Pyc.hu, human Pyc (11); Pyc.rat, rat Pyc (19); PCC.rat, rat propionyl coenzyme A carboxylase (6); BC.Ec, E. coli acetyl coenzyme A carboxylase (24); Pyc.Re, R. etli (9). Conserved amino acid sequences (boxed) were used to design degenerate oligonucleotide primers for PCR.