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. 2022 May 30;12:885480. doi: 10.3389/fonc.2022.885480

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Copyright © 2022 Ge, Song, Engelbach, Yuan, Gao, Dahiya, Rich, Ackerman and Garbow

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-course plots (means ± SEM) for the concentration of (left) Lac and (middle) Glx, and (right) the concentration ratio Glx : Lac derived from a 90-min series of 10-min acquisition spectra with n = 4 mice each condition (control, tumor-bearing, radiation necrosis). These time-course spectroscopy data were analyzed using two-way ANOVA for repeated measurement, followed by post-hoc comparison of the least squares means (LSM) across groups. ANOVA2/LSM results are—Lac: tumor vs. necrosis, p <0.0001; tumor vs. control, p <0.0001; necrosis vs. control, p = 0.7631; Glx: tumor vs. necrosis, p = 0.0671; tumor vs. control, p = 0.0004; necrosis vs. control, p = 0.0685; Glx/Lac: tumor vs. necrosis, p <0.0001; tumor vs. control, p <0.0001; necrosis vs. control, p <0.0001.