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. 2022 May 27;13:888961. doi: 10.3389/fphar.2022.888961

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Copyright © 2022 Conzelmann, Muratspahić, Tomašević, Münch and Gruber.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of plant extracts on HIV-1 infection of TZM-bl cells. Ten peptide-enriched plant extracts were obtained using solvent and solid phase extraction, and their molecular content was analyzed by MALDI-TOF MS (Figure S1). Extracts were dissolved in 5% DMSO. Serial dilutions of extracts #1 to #10 and DMSO control were added to TZM-bl cells, which were then infected with HIV-1 (infection, blue) or medium was added (metabolic activity, grey). After 2 days, infection and metabolic activity was quantified via β-galactosidase or MTT-based assay, respectively. Untreated controls were set to 100%. Shown are means ± SD of one (#10, metabolic activity), two (#10, infection) or three (all others) independent experiments performed in triplicates.