TABLE 3.
Overview about tested Viola tricolor cyclotide subfractions.
| Fraction # | Cyclotides | Mass monoiso. (m/z) | Retention time (min) | Purity (%) |
|---|---|---|---|---|
| 4.1 | vigno 5 | 2,858.8 | 46.67 | >70 |
| acyclic vitri E, acyclic cO22, cO28 | 2,922.8 | |||
| 4.2 | vitri peptide 2, vigno 9, cO2 | 3,138.9 | 44.20 | >80 |
| 4.3 | kalata S, varv C/D, acyclic vigno 5 | 2,876.7 | 47.65 | >90 |
| 4.4 | kalata B1, varv E, vigno 3 | 2,890.6 | 48.77 | ∼50 |
| vigno 4, chacur 1, cO22 | 2,904.6 |
Cyclotides in each subfraction were identified by molecular weight obtained by MALDI-TOF MS, as compared to CyBase entries for Viola tricolor (Wang et al., 2007).