Fig 2. Fluorescence melting peaks obtained by plotting the negative derivative of fluorescence over temperature (-dF/dT) versus temperature (T) in the SYBR Green channel (465–510 nm) for the SYBR Green qPCR multiplex.

Each gene combination observed in this study is represented here: Combination 1—susceptible to three classes of antibiotics, only pbp2b gene was positive; 2—susceptible to penicillin and resistant to clindamycin and erythromycin, pbp2b and ermB genes were positive; 3—resistant to penicillin and susceptible to clindamycin and erythromycin, all three genes were negative; 4—resistant to three classes of antibiotics, with multiples mechanisms of resistance, pbp2b was negative, ermB and mef genes were positive; 5—resistant to penicillin, clindamycin and erythromycin, ermB gene was positive. The peaks were identified with the name of the genes that they represent.