Table 1. Statistic analyses of melting temperature (Tm) values obtained in the dissociation curve after qPCR using DNA purified from cultivated strain and purified from cerebrospinal fluid (CSF) sample.
pbp2b | ermB | mef | ||||||
---|---|---|---|---|---|---|---|---|
Strain | CSF (silica) | CSF (heating) | Strain | CSF (silica) | CSF (heating) | Strain | CSF (silica and heating) | |
Number of values | 39 | 11 | 8 | 11 | 4 | 3 | 3 | 3 |
Minimum | 78.22 | 77.98 | 79.39 | 81.28 | 80.81 | 82.07 | 75.19 | 74.85 |
Maximum | 79.03 | 78.83 | 79.90 | 81.51 | 81.19 | 82.56 | 75.23 | 76.34 |
Mean ± SD | 78.74 ±0.13 | 78.42 ±0.23 | 79.69 ±0.21 | 81.36 ±0.07 | 81.06 ±0.17 | 82.27 ±0.26 | 75.21 ±0.02 | 75.38 ±0.83 |
IQR 25–75 | 78.67–78.81 | 78.24–78.53 | 79.44–79.86 | 81.30–81.40 | 80.88–81.18 | 82.07–82.56 | 75.19–75.23 | 74.85–76.34 |
Normality test | No | Passed | Passed | Passed | Too small a | Too small a | Too small a | Too small a |
Coefficient of variation | 0.16% | 0.29% | 0.26% | 0.09% | 0.21% | 0.32% | 0.02% | 0.97% |
One sample t test (P-value) | < 0.0001 | < 0.0001 | 0.0387 | < 0.0001 | 0.0016 | 0.2194* | < 0.0001 | 0.0779* |
Acceptance criteria | 78.24–79.86 | 80.88–82.56 | 74.85–76.34 |
Strain: DNA purified from isolated strains; CSF silica: DNA purified from CSF samples using silica column; CSF heating: DNA purified from CSF samples by heating; number of values: Number of different samples tested for each condition; Minimum: The smallest value of Tm obtained; Maximum: The largest value of Tm obtained; Mean ± SD: Mean and the standard deviation; IQR 25–75: The interquartile range of the 25th and 75th percentile; Normality test: Results obtained from AP test and SW test to check normal distribution. Passed implies P-value > 0.05, which means that the data are consistent with a Gaussian distribution; Coefficient of variation: The standard deviation divided by the mean; One sample t test: P-value obtained from comparison of the mean to the hypothetical value estimated by uMELT; Acceptance criteria: The acceptance Tm range suggested in this study.
a considered normal distribution by the KS normality test.
* No significant difference to the hypothetical value was observed.