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. 2022 Jun 14;17(6):e0269895. doi: 10.1371/journal.pone.0269895

Table 1. Statistic analyses of melting temperature (Tm) values obtained in the dissociation curve after qPCR using DNA purified from cultivated strain and purified from cerebrospinal fluid (CSF) sample.

pbp2b ermB mef
Strain CSF (silica) CSF (heating) Strain CSF (silica) CSF (heating) Strain CSF (silica and heating)
Number of values 39 11 8 11 4 3 3 3
Minimum 78.22 77.98 79.39 81.28 80.81 82.07 75.19 74.85
Maximum 79.03 78.83 79.90 81.51 81.19 82.56 75.23 76.34
Mean ± SD 78.74 ±0.13 78.42 ±0.23 79.69 ±0.21 81.36 ±0.07 81.06 ±0.17 82.27 ±0.26 75.21 ±0.02 75.38 ±0.83
IQR 25–75 78.67–78.81 78.24–78.53 79.44–79.86 81.30–81.40 80.88–81.18 82.07–82.56 75.19–75.23 74.85–76.34
Normality test No Passed Passed Passed Too small a Too small a Too small a Too small a
Coefficient of variation 0.16% 0.29% 0.26% 0.09% 0.21% 0.32% 0.02% 0.97%
One sample t test (P-value) < 0.0001 < 0.0001 0.0387 < 0.0001 0.0016 0.2194* < 0.0001 0.0779*
Acceptance criteria 78.24–79.86 80.88–82.56 74.85–76.34

Strain: DNA purified from isolated strains; CSF silica: DNA purified from CSF samples using silica column; CSF heating: DNA purified from CSF samples by heating; number of values: Number of different samples tested for each condition; Minimum: The smallest value of Tm obtained; Maximum: The largest value of Tm obtained; Mean ± SD: Mean and the standard deviation; IQR 25–75: The interquartile range of the 25th and 75th percentile; Normality test: Results obtained from AP test and SW test to check normal distribution. Passed implies P-value > 0.05, which means that the data are consistent with a Gaussian distribution; Coefficient of variation: The standard deviation divided by the mean; One sample t test: P-value obtained from comparison of the mean to the hypothetical value estimated by uMELT; Acceptance criteria: The acceptance Tm range suggested in this study.

a considered normal distribution by the KS normality test.

* No significant difference to the hypothetical value was observed.