Table 2. Antimicrobial sensitivity profile and genotypes of clinical isolates using SYBR Green qPCR multiplex.

No. of isolates	Susceptibility profile			Presence of genes
	Penicillin	Clindamycin	Erythromycin	pbp2b	ermB	mef
34	Susceptible	Susceptible	Susceptible	34a	-	-
6	Susceptible	Resistant	Resistant	5b	6	-
6	Resistant	Susceptible	Susceptible	-	-	-
4	Resistant	Resistant	Resistant	-	4	2*
1	Resistant	Susceptible	Resistant	-	1c	1*
51				39	11	3

qPCR with the discordant result:

^a, for strain 357, the pbp2b gene is not amplified according to the acceptance criteria, and it presents susceptibility to penicillin;

^b, for strain 404, the pbp2b gene is not amplified, but it is susceptible to penicillin;

^c, for strain 444, ermB gene is positive, but the strain is susceptible to clindamycin.

This strain is also erythromycin-resistant and has both genes (ermB and mef).

* Total of three strains presented both ermB and mef genes.