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. 2022 May 30;12:817192. doi: 10.3389/fonc.2022.817192

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Copyright © 2022 Li, Cao, Zhao, Li, Xu, Cui, Deng, Gao and Wei

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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circDNMT1 knockdown inhibits proliferation, migration and invasion of GC cells. (A) The qRT-PCR analysis to show circDNMT1 expression in HGC-27 and AGS cells stably carrying lentivirus with NC shRNA or circDNMT1 shRNA and additionally transfected with vector or circDNMT1 overexpression plasmids. (B, C) The CCK-8 assay to show proliferation of cells as in (A). (D) The EdU assay to show proliferation of cells as in (A). Scale bar: 100 μm. The histogram is displayed on the right. (E) The wound healing assay to show migration of cells as in (A). The histogram is displayed on the right. (F) The transwell assay to show invasion of cells as in (A). The histogram is displayed on the right. Scale bar: 100 μm. Data were presented as means ± SD. *P < 0.05, **P < 0.01.