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. 2022 May 30;12:817192. doi: 10.3389/fonc.2022.817192

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Copyright © 2022 Li, Cao, Zhao, Li, Xu, Cui, Deng, Gao and Wei

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-576-3p is directly sponged by circDNMT1 in GC. (A) The schematic illustration of miRNAs that can be sponged by circDNMT1 predicted by Circinteractome. (B, C) The qRT-PCR analysis to show the miRNA levels that were pulled down by NC probe or circDNMT1 probe in HGC-27 (B) and AGS (C) cells. (D) The schematic illustration of wildtype (red) and mutant (green) sequences of binding sites of circDNMT1 and miR-576-3p. (E, F) Luciferase reporter assay to show the relative luciferase activities in HGC-27 (E) and AGS (F) cells that were cotransfected with empty luciferase reporter plasmids (vector) or plasmids inserted with wildtype or mutant sequences and NC and miR-567-3p mimics. **P < 0.01, ***P < 0.001.