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. 2022 May 27;13:903310. doi: 10.3389/fpls.2022.903310

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Copyright © 2022 Li, Cao, Zhang, Lv and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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qRT-PCR analysis of rmyA and rmyB in wide type strain EP1, phcA deletion mutant (ΔphcA), and complementary strain ΔphcA(phcA) (OD₆₀₀ = 1.0). The results were calculated as 2^–ΔΔCt, –ΔΔCt = −(ΔCt_mutant –ΔCt_wt). Three biological repeats (independent cultures) and three technical repeats were done to calculate and compare the values. Mean values of all experiments were averaged with SD (error bars) by using the GraphPad Prism 6.0 software.