Figure 6.

Schematic presentation of rmyA and rmyB operons (A), proteins (PhcA and PhcR) expression (B) and promoter binding detection by electrophoretic mobility shift assay (E,F). 30 fmol of labeled DNA fragments corresponding to the promoter region of rmyA gene was incubation with 100 nm and 200 nm PhcA/R, respectively, using 100-fold unlabeled corresponding DNA fragments as the specific competitor. The coding sequence of impH (424 bp), which encodes a type VI secretion protein and is not bound by PhcA and PhcR, was used as a negative control in the EMSA experiment (C,D). The positions of free DNA and of protein–DNA complexes were shown.