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. Author manuscript; available in PMC: 2022 Jun 14.
Published in final edited form as: Chem Rev. 2022 Mar 22;122(8):7690–7719. doi: 10.1021/acs.chemrev.1c00857

Figure 8. Subunit Exchange between sHSP Oligomers Illuminated through MS.

Figure 8.

A) Two sHSP homo-oligomers (R and B, numbers indicated the number of subunits within the example oligomers) with suspected hetero-oligomeric interactions are co-incubated in solution, and MS data is collected at various time points (t1, t2, t3 etc.). B) The MS data is quantified versus time to measure the decay of homo-oligomers and the growth in abundance of hetero-oligomeric sHSP species. The rate of this conversion from homo-oligomers to hetero-oligomers can be measured as a function of time, and can serve as an indication or stability. C) When hetero-oligomeric sHSP complexes are stable their subunit exchange is expected to occur quickly. D) Conversely, when hetero-oligomeric complexes are unstable, the equilibrium shifts in the direction of homo-oligomers.