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. Author manuscript; available in PMC: 2023 Feb 4.
Published in final edited form as: Chembiochem. 2021 Nov 29;23(3):e202100559. doi: 10.1002/cbic.202100559

Figure 2.

Figure 2.

Effects of single-entity oligosaccharides and the HMO cocktail at ca. 5 mg/mL on growth and viability of S. salivarius (ATCC 19258) and S. agalactiae (GB00002). Growth was quantified via OD600 readings at 0, 2, 4, 6, 7, 8, and 24 h. Mean OD600 for each time point is indicated by the corresponding symbols. Viability was assessed by enumeration of CFU/mL performed at 0, 2, 4, 6, 7, 8, and 24 h for S. salivarius and 0, 4, 7, and 24 h for S. agalactiae. Log10 CFU/mL for each HMO and time point is designated by the corresponding symbols. (A) Growth of S. salivarius (OD600) in the presence of single-entity oligosaccharides and the HMO cocktail. (B) Viability of S. salivarius (CFU/mL) corresponding to the OD values graphed in Figure 2A. (C) Growth of S. agalactiae (OD600) in the presence of single-entity oligosaccharides and the HMO cocktail. (D) Viability of S. agalactiae (CFU/mL) corresponding to the OD values graphed in Figure 2C. Data displayed represent the relative mean growth ratios ± SEM of three independent experiments, each with three technical replicates. In (A) ∗∗∗∗ represents p < 0.0001, *** represents p = 0.0009 and p = 0.0003, and * represents p = 0.0132 by two-way ANOVA with post hoc Dunnett’s multiple comparison test comparing the growth of S. salivarius in each oligosaccharide supplementation condition to the growth of S. salivarius in medium alone. In (B) ∗∗∗∗ represents p < 0.0001, *** represents p = 0.0005, ** represents p = 0.0085, p = 0.0062, and p = 0.0022, and * represents p = 0.0484 by two-way ANOVA with post hoc Dunnett’s multiple comparison test comparing the growth of S. salivarius in each oligosaccharide supplementation condition to the growth of S. salivarius in medium alone. In (C) ∗∗∗∗ represents p < 0.0001, *** represents p = 0.0009 and p = 0.0007, and ** represents p = 0.0015 by two-way ANOVA with post hoc Dunnett’s multiple comparison test comparing the growth of S. agalactiae in each HMO supplementation condition to the growth of S. agalactiae in medium alone. In (D) *** represents p = 0.0010, p = 0.0005, and p = 0.0002, and ** represents p = 0.0037 by two-way ANOVA with post hoc Dunnett’s multiple comparison test comparing the growth of S. agalactiae in each oligosaccharide supplementation condition to the growth of S. agalactiae in medium alone.