Figure 4.

Suppression of S. salivarius by S. agalactiae is combatted when the supernatants from overnight cultures are treated with DNAase I, lipase, α-amylase, proteinase K, or 1 mM TRIS buffer. Supernatants from overnight solo cultures and cocultures are treated with enzyme or TRIS buffer to determine if the suppression of S. salivarius by S. agalactiae is reversed. Cultures with GOS supplementation were added at ca. 5 mg/ml. Growth was quantified via OD₆₀₀ readings at 24 h. Data displayed is a combined from treatments with all four enzymes and TRIS buffer. Data displayed represent the relative mean growth ratios ± SEM of three independent experiments, each with three technical replicates.