Figure 5.

S. agalactiae in medium alone produces significantly more lactic acid compared to all other conditions tested. The first four bars represent controls in which S. salivarius (ATCC 19258) and S. agalactiae (GB00002) were grown separately, either with or without GOS supplementation at ca. 5 mg/ml. The last four bars represent the two strains grown in coculture either with or without GOS supplementation at ca. 5 mg/ml. Lactic acid concentration was quantified via OD₄₅₀ readings at 24 h. Data displayed represent the relative mean growth ratios ± SEM of three independent experiments, each with three technical replicates. **** represents p < 0.0001 by one-way ANOVA with post hoc Dunnett’s multiple comparison test comparing growth in S. agalactiae in medium alone to growth in all other conditions.