Figure 7.

In coculture, GOS and galactose supplementation assist in circumnavigating the suppression of S. salivarius by S. agalactiae. Growth of S. salivarius (ATCC 19258) and S. agalactiae (GB00002) supplemented with ca. 5 mg/ml of GOS, galactose, glucose, and lactose were compared to growth of S. salivarius and S. agalactiae grown in THB medium alone. Growth was quantified via OD600 24 h. Data displayed represent the relative mean growth ratios ± SEM of three independent experiments, each with three technical replicates. **** represents p < 0.0001, *** represents p = 0.0006, ** represents p = 0.0099 and p = 0.0085 by one-way ANOVA with post hoc Dunnett’s multiple comparison test comparing growth in carbohydrate-supplemented Todd-Hewitt Broth (THB) to growth in carbohydrate-free THB.