Figure 3.
Example output (DatLab software by Oroboros, Innsbruck, Austria) from a HRR experiment on the Oxygraph-2k. The y-axes show O2 flux per mass (red line; pmol · s−1 · mg−1) and oxygen (O2) concentration (blue line). The SUIT protocol for this study was as follows: pyruvate + malate + glutamate (LEAK respiration), ADP to determine oxidative capacity (OXPHOS) with CI (PCI), addition of cyt c, succinate to determine OXPHOS with CI and CII (PCI+II), titrations of carbonyl cyanide m-chlorophenyl hydrazone (CCCP) to determine ETS capacity with CI and CII (ECI+II), rotenone to determine ETS capacity with CII (ECII), and antimycin A to determine residual oxygen consumption (ROX).