FIGURE 5.

Plasma levels of sulfur amino acids are similar for mice subjected to IMR and continuous MR. Comparisons of the relative plasma levels of (a) methionine and (b‐c) homocysteine are shown for female (a‐b) and male (c) mice that were control‐fed (CF), continuously methionine‐restricted (MR), or subjected to stringent IMR (IMR2). IMR2 values represent sampling following either a period of methionine restriction (RES) or methionine repletion (REP). Prior to sampling, animals were fed the indicated diets for ~18 weeks (a‐b) or ~6 weeks (c). Bars denote SEM. Statistically significant differences (as compared with the corresponding CF values) are indicated (**p < 0.01; ***p < 0.001; ****p < 0.0001). Statistically significant differences between MR and IMR2 (RES) values are either indicated (*p < 0.05; ***p < 0.001) or absent (ns). For panels a‐b, N=8 for all groups; for panel c, N = 4 for all groups