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. 2022 Jun 15;132(12):e153580. doi: 10.1172/JCI153580

Figure 2. Autoreactive ASCs with high and low affinity are present in the spleen, blood, and bone marrow (BM) of patients with ITP.

Figure 2

(A) IgG secretion rate and (B) affinity for αIIbβ3 of single ASCs from pooled data of patients with ITP and healthy donors (HD) for spleen (ITP, n = 14; HD, n = 6), BM (ITP, n = 17; HD, n = 4), and blood (ITP, n = 14; HD, n = 10). Single-cell values and medians are plotted. Total numbers of IgG-SCs analyzed per compartment are indicated in A. In B, affinities are classified into high (red), medium (orange), and low (yellow) affinity, with dotted lines separating these categories. (C) Frequency of αIIbβ3-reactive IgG-SCs among mononuclear cells (MCs) classified into high, medium, and low affinity from patients (ITP) and HD according to B. (D–F) Frequency of αIIbβ3-reactive IgG-SCs among MCs classified into high, medium, and low affinity represented for individual patients (ITP) and HD for (D) spleen, (E) BM, and (F) blood. (D–F) The dotted line marks the highest frequency found in an HD. **P < 0.01; ****P < 0.0001 using post hoc contrast analysis after linear (A and B) or logistic (C) modeling and multiple-testing P-value adjustment. NS, not significant. See Supplemental Table 2 for further details.