Figure 7. JAB0505 treatment causes a sustained increase in several immune cell populations following skeletal muscle injury of Acvr1^tnR206H/+; Tie2-Cre FOP mice.

Flow cytometry analysis was used to determine cell numbers of (A) total CD45⁺ hematopoietic cells, (B) myeloid cells, (C) lymphoid cells, (D) total macrophages, (E) Ly6C⁺ inflammatory monocytes/macrophages, (F) neutrophils, (G) mast cells, and (H) T cells in injured distal hind limb muscles of control (R26^NG/+; Tie2-Cre) and FOP (Acvr1^tnR206H/+; R26^NG/+; Tie2-Cre) mice that were either untreated or administered 10 mg/kg JAB0505 i.p. (n = 3–4). Error bars represent ±SD. Significance was determined using 1-way ANOVA with Tukey’s multiple-comparison test within individual time points. Symbols representing significance were placed above FOP and FOP + JAB0505 bars to indicate a comparison to control (^), control + JAB0505 (^#), and FOP (*). The numbers of symbols of each type denote levels of significance: (1) P ≤ 0.05, (2) P ≤ 0.01, (3) P ≤ 0.001, and (4) P ≤ 0.0001. No control vs. control + JAB0505 comparisons were significant.