Figure 1.

ASCs promote the viability, proliferation, and colony formation of breast cancer cells following the release of cytokines and chemokines. (a–c) Cell viability, proliferation, and colony formation were analyzed using CCK-8 assays, dsDNA quantification, and colony formation assays in MDA-MB-231 cells treated with culture supernatant from ASCs. (d–f) The release of cytokines and chemokines including SCF, IL-6, sCD31, MCP-1, MIP-1α, SDF-1, VEGFA, and TNFα in MDA-MB-231 cells cocultured with ASCs was analyzed using Milliplex MAP kit. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; NS: no significance.