Figure 2.

The effects of ASCs on MDA-MB-231 breast cancer cells are mediated by MEK-ERK-CBP signaling. (a) Western blot analysis of the association between MAPK pathways and CBP in MDA-MB-231 cells treated with ASC culture supernatant. (b–c) The indicated proteins in MDA-MB-231 cells induced by ASCs and the cell viability (d) and proliferation (e) were analyzed. The cells were pretreated with IgG- or anti-SCF-neutralizing antibody for 1 h, 1 μM selumetinib (MEKi), and 10 μM SP600125 (JNKi) for 24 hours. The results were normalized; the cells that were treated with IgG were used as a positive control; and the value was set to 1. ∗P < 0.05, ∗∗P < 0.01.