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. 2022 Jun 7;2022:8540535. doi: 10.1155/2022/8540535

Figure 3.

Figure 3

CBP coordinated with RFPL-3 to coregulate hTERT transcriptional activity in breast cancer cells. (a) MDA-MB-231 cells were treated with ASC culture supernatant, and then, the expression levels of RFPL-3 and hTERT protein were analyzed by western blot. (b) The localization of CBP and RFPL-3 in MDA-MB-231 cells was confirmed by confocal microscopy. (c) MDA-MB-231 cells were cotransfected with CBP vectors and nonspecific siRNA or RFPL-3 siRNA, and then, the expression levels of CBP, RFPL-3, and hTERT were detected using western blot. (d) MDA-MB-231 cells with CBP upregulation were co-transfected with RFPL-3 siRNA or control siRNA and hTERT-luciferase plasmids, and the relative luciferase activity was examined. (e) MDA-MB-231 cells were co-transfected with RFPL-3 vectors and CBP-specific siRNA or nonspecific siRNA, and then, the expression levels of RFPL-3, CBP, and hTERT were evaluated by western blot. (f) CBP-specific siRNA and hTERT promoter-driven luciferase plasmids were co-transfected into MDA-MB-231 cells overexpressing RFPL-3, and then, the relative luciferase activity was analyzed in the cells.∗∗P < 0.01, ∗∗∗P < 0.001.