Figure 3.

C. neoformans stimulates IL-17 production by CD4⁺ T cells in vitro. Peripheral blood mononuclear cells were stimulated with C. neoformans or bacillus Calmette–Guérin for 3 days with protein transport inhibitor cocktail for 5 h before the cells were collected. The IL-17-producing cells were analyzed by flow cytometry: representative data of IL-17⁺ T cells in αβTCR⁺ T cells (CD3⁺γδTCR^- cells) and γδTCR⁺ T cells (A) and percentages of IL-17⁺ CD3⁺ cells in live lymphocytes and percentages of IL-17⁺ CD4⁺ cells in live CD3⁺ cells (B). The corresponding IL-17 level in the supernatant (C) is shown as mean ± SE of three individual experiments. ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.