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Polarization of the IgG subclass response is illustrated as ratio of IgG2a over IgG1 titers at day 35 (A). OVA-specific proliferation of splenocytes from transcutaneous immunized mice (B). OVA-specific IFN-γ (C) or IL-2 (D) secretion of splenocytes from immunized mice. The data were presented as mean ± sd (n = 6), *P＜0.05, **P＜0.01, ***P＜0.001.
