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. 2022 Jun 14;9:335. doi: 10.1038/s41597-022-01380-9

Fig. 1.

Fig. 1

Graphical representation of the DIA data reanalysis pipeline, consisting of 4 parts. (a) Data curation: Metadata annotation protocols and dataset acquisition. (b) SWATH-MS data analysis: Nextflow workflow including steps ranging from data conversion, SWATH-MS window management, data quality assessment and control (QA/QC), OpenSWATH analysis, FDR calculation to measurement alignment. (c) Statistical analysis: Nextflow workflow for MSstats analysis, normalisation and result filtering. (d) Data integration: Data preparation, accession mapping and integration into Expression Atlas.