Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 14;13:3422. doi: 10.1038/s41467-022-30480-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A Relationship between flow cytometry samples. gRNA #48U is a weaker version of #48. N = 4–6 biologically independent replicates. B Relationship between clones based on their indel profiles, analogous to Fig. 2B. Only non-significant clones are shown for clarity. Biological replicates (N = 2) were averaged. C Difference in indel frequency between the regular gRNA #48 and its less active counterpart #48U. Same display conventions as in Fig. 2. D Relationship between clones based on their indel profiles, analogous to Fig. 2B. Each clone is colored by the frequency of mutagenesis assayed by flow cytometry on day 14 using gRNA #48U, a proxy for Cas9 activity. For clarity, only non-significant clones are depicted.