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. 2022 Jun 14;13:3433. doi: 10.1038/s41467-022-30977-2

Fig. 7. Birthdate-dependent targeting of morphological MLI subtypes by Ascl1-CreER.

Fig. 7

a MLI labeling strategy by tamoxifen (TMX) inducible Ascl1-CreERT2 and fluorescent Cre reporters, Ai14-TdTomato (cytosolic) or mTmG (membrane-targeted). b Schematic for TMX-induced subtype-enriched MLI labeling, with postnatal time points of single dose TMX injections (oil droplets) and collection (arrows). Injection at P0 predominantly labels BC populations (early-born, pink), while P4-7 injections label SCs in the upper ML (late-born, teal). c Confocal images of cerebellar sections from P25 Ascl1-CreER; Ai14-TdTomato mice with TMX induction at P0, P4, or P7. d P0-induced BCs in the lower ML. e P3-induced SCs in the mid-upper ML with descending basket terminals. Pink asterisks denote basket formations enveloping PC soma in c–e. f P0-induced non-basket forming SC in the lower ML. Representative images were taken at P25. N = 3–5 animals per injection time point (c–f). Scale bars are 50 µm.