Table IV.

Particle Size Distribution of Spray-Dried RALA/pEGFP-N1 NPs. Approximately 1 mg of spray-dried RALA/pEGFP-N1 NPs were suspended in chloroform in a 50 ml glass cuvette and were stirred with a magnetic bar at 1000 rpm. Subsequently, a 60 s of sonication at a power of 60 W was performed before measurement

Spray-dried RALA NPs	D10 (µm)	D50 (µm)	D90 (µm)	Span
2% mannitol	1.56 ± 0.34	3.18 ± 0.78	7.85 ± 1.95	2.05 ± 0.55
3% mannitol	1.20 ± 0.40	3.17 ± 0.06	7.03 ± 0.94	1.85 ± 0.34
5% mannitol	1.46 ± 0.05	3.05 ± 0.31	8.04 ± 2.45	2.24 ± 1.01

Dx: particle size distribution at x% of the volume distribution; Span, width of the volume distribution relative to the median diameter (D₉₀—D₁₀)/D₅₀.