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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: Anaerobe. 2022 Mar 31;75:102555. doi: 10.1016/j.anaerobe.2022.102555

Figure 2. Western blots of purified CI_01447 and CI_01448 probed with anti-toxin A and B antibodies.

Figure 2.

Representative immunoblots performed with purified CI_01447 and CI_01448 probed with (A) C. difficile anti-toxin A antibody (ACdTA-HRP), and (B) C. difficile anti-toxin B antibody (ACdTB-HRP). (C-D) Coomassie stained SDS-gel were used as loading controls. All experiments were performed in at least biological duplicate; purified C. difficile TcdA (308 kDa) and TcdB (270 kDa) were used as positive controls. Arrows identify protein bands interacting with C. difficile anti-toxin A and B antibodies at ~40 kDa. A total of 50 ng of recombinant C. innocuum proteins and 1 ng of TcdA or TcdB was loaded.